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KMID : 0545119930030020086
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Journal of Microbiology and Biotechnology
1993 Volume.3 No. 2 p.86 ~ p.90
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Cloning and DNA Sequence of Carboxymethylcellulase(CMCase) Gene from Cellulomonas sp. YE-5
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HER, SONG
KIM, DONG SEOB/CHOI, SUN JIN/OH, DOO HWAN
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Abstract
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CMCase positive clones were screened from Cellulomonas sp. YE-5 and named pCE1, pCE2 and pCE3. Among the positive clones pCE1 was used for this study, because it has the smallest insert and the highest CMCase activity among the 3 clones, and its nucleotide sequence was determined. The CMCase gene in pCE1 was composed of 1071 by of nucleotides coding 357 amino acids. Computer analysis showed that the pCE1 has 65% sequence homology with the endoglucanase from Cellulomonas fimi.
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